THE INFLUENCE OF LONG-TERM TAKING A VITAMIN-MINERAL COMPLEX IRON CITRATE (SYNTHESIT® Iron) ON MICE BONE MARROW CELL INDICATORS

D. V. Bulgin (1), V. А. Karabanov (2), R. V. Karabanov (2)
1 Federal State Budget Scientific Institution "Research Institute of Medical Primatology", Sochi
2 LLC"SynthestechResearch Center", Sochi


The safety and efficiency of long-term taking this vitamin-mineral complex based on iron citrate were explored in laboratory SHK-line mice. It has been proven that iron citrate does not have a toxic (damaging) effect on the internal organs and tissues of mice. The basic cellular parameters of the bone marrow were studied. The effect of iron citrate on the intensity and dynamics of cell populations proliferative activity in all sprouts of the bone marrow hematopoiesis was revealed.
Iron citrate is not toxic (damaging) effects on the internal organs and tissues of mice
Liver tissue of a typical histological structure
(hematoxylin and eosin coloring, x400 scaling)
Myocardial tissue of a typical histological structure
(hematoxylin and eosin coloring, x400 scaling)
Kidney tissue of a typical histological structure
(hematoxylin and eosin coloring, x400 scaling)
Lung tissue of a typical histological structure
(hematoxylin and eosin coloring, x400 scaling)
It is known that the main source of stem cells (SC) is bone marrow (BM). The phenomenon of remote control of BM over the processes of tissue and organ regeneration has been scientifically proven, and SC BM are directly involved in these processes.

Bone marrow isthe foundation of stem cells and progenitor cells.

The cellular composition of BM is heterogeneous in origin and morphology; BM cells are represented at different stages of their development, among them are mononuclear BM cells (bone marrow mononuclear cells / BMMNCs).

All BM cells that have one nucleus and do not contain granules in the cytoplasm are commonly referred to the BMMNCs population. Among BMMNCs, there is a big amount of cells - hematopoietic precursors at different stages of maturation, lymphocytes, plasma cells, monocytes.

The BMMNCs fraction is an extensive source of cells that can be used in regenerative medicine and tissue engineering technologies.
The number of cellular elements of all germs of hematopoiesis is within normal limits
Bone marrow of a mouse not taking iron citrate
(hematoxylin and eosin coloring, x400 scaling)
The number of cellular elements of all germs of hematopoiesis isincreased
Bone marrow of a mousetaking iron citrate
(hematoxylin and eosin coloring, x400 scaling)
THE CONTROL
A smear imprint of the bone marrow of a mouse not taking iron citrate
(May-Grunwald-Giemsa coloring, cytological micropreparation, scaling x400)
THE EXPERIMENT
A smear imprint of the bone marrow of a mouse taking iron citrate
(May-Grunwald-Giemsa coloring, cytological micropreparation, scaling x400)
THE CONTROL
A smear imprint of the bone marrow of a mouse not taking iron citrate
(May-Grunwald-Giemsa coloring, cytological micropreparation, scaling x1000)
THE EXPERIMENT
A smear imprint of the bone marrow of a mouse taking iron citrate
(May-Grunwald-Giemsa coloring, cytological micropreparation, scaling x1000)
GENERAL INDICATORS OF BONE MARROW IN MICE EXPERIMENTAL AND CONTROL GROUPS
Note:
* -statistically significant difference compared with the control group.
Conclusions
1. Myelogram changes in the experimental and control groups indicate that prolonged oral taking of the SYNTHESIT® Iron not only enhances hematopoiesis, but also increases its reparative processes in the red bone marrow due to an increase in the number of mononuclear (stem) cells.

2. Iron citrate, included in the SYNTHESIT® Iron does not have toxic (damaging) effects on the internal organs and tissues of mice with prolonged oral taking.